Fig. 2

Representative images of organoids cultured from a mammary Paget’s disease patient. a Surgical specimen of mammary Paget’s disease. b Cut an appropriate size of nipple tumor tissue and breast tumor tissue behind the nipple for culture and store respectively. c Postoperative pathology results reported that both the nipple area and the mass behind the nipple were adenocarcinoma. d, e Culture tumor cells and organoids using nipple tumor tissue and breast tumor tissue under 2D and 3D cultivating mode separately