Fig. 1
From: Transgelin interacts with PARP1 in human colon cancer cells
The localization of transgelin in different human colon cancer cell lines. a Transgelin in colon cancer cell lines RKO, SW480, HCT116 and LOVO was observed via immunofluorescence. The panels show transgelin immunostaining (red), 4′,6-diamidino-2-phenylindole DNA staining (DAPI), and a merged image is shown. b The distribution of transgelin in SW480, LOVO, HCT116 and RKO cells were identified via immunoblotting. c The distribution of transgelin-flag fusion protein in RKO and SW480 cells that were transiently transfected with pcDNA6/myc-His B-TAGLN-flag plasmid and control plasmid as detected via immunoblotting. N refers to the nuclear protein fraction, and C is the cytoplasmic protein fraction. Lamin B1 is a nuclear protein marker, GAPDH is a cytoplasmic protein marker