Fig. 4

Prediction of the transcription factor(s) of the key genes and validation of transgelin-PARP1 interaction. a An illustration of the computational model for predicting the transcription factors of the key genes. b The prediction of the transcription factors of the key genes (partially illustrated). PARP1 protein is circled in a blue frame. c The recommendation degree of PARP1 as the transcription factor of the seven key genes downstream of transgelin. d The sequence of PARP1 protein. The red fonts represent the corresponding sequences of possible nuclear localization signal in PARP1 protein. e Immunoblotting analysis of transgelin and flag protein expression in RKO-TAGLN-FLAG, RKO-CTRL and wild type RKO cells, ****P < 0.0001. f Interaction between the transgelin-flag fusion protein and PARP1 was validated by co-immunoprecipitation. RKO-CTRL was the control group and normal rabbit IgG was used as the control antibody. g Immunofluorescence analysis of transgelin and PARP1 in RKO cells. The panels show transgelin immunostaining (green), PARP1 immunostaining (red), 4′,6-diamidino-2-phenylindole DNA staining (DAPI), and a merged image is indicated