Fig. 2

Effect of the combination treatment on HCC cell apoptosis. Synergistic activity of the combination treatment of biochanin A and SB590885 on apoptosis after 48 h of exposure in the HCC cell lines a Bel-7402 and b SK-Hep1 cell line. The results are the mean ± SD of three independent experiments performed in triplicate. **P < 0.001 versus single treatments (one-way ANOVA followed by a Student–Newman–Keuls test). BCL2, BAX, cleaved caspase-9 and cleaved PARP expression was measured in the c Bel-7402 cell line d, and SK-Hep-1 cell line using western blot analysis