Fig. 5

YY1-mediated OIP5-AS1 promotes HCC cell growth by activating WNT pathway. a Expression of OIP5-AS1 in YY1-silenced HCC cells was detected by qRT-PCR. b qRT-PCR was carried out to measure OIP5-AS1 expression in HCC cells upon YY1 upregulation. c The binding motif of YY1 and the binding site between YY1 and OIP5-AS1 promoter were predicted by JASPAR. d ChIP assay was conducted to determine the interaction between YY1 and OIP5-AS1 promoter. e The interaction between YY1 and OIP5-AS1 promoter was further verified by luciferase reporter assay. f, g The effect of OIP5-AS1 silencing on the mRNA and protein levels of Axin2, CTNNB1 (non-phosphorylated β-catenin and phosphorylated β-catenin), Cyclin D1 and c-myc was detected by qRT-PCR and western blot analyses. h, i The nuclear translocation of β-catenin was assessed by nuclear-cytoplasmic fractionation followed by western blot analysis in two OIP5-AS1-downregulated HCC cells. j The effect of OIP5-AS1 depletion on the activity of WNT pathway in HepG2 and MHCC97H cells was determined with the employment of TOP/FOP-flash luciferase reporter assay. k The proliferation of HCC cells transfected with sh-NC or sh-OIP5-AS1 when exposed to CHIR99021 was estimated by colony formation assay. l The apoptosis of indicated cells was examined by caspase-3 activity analysis. **P < 0.01