Fig. 2

Tan IIA induces miR-497-5p expression in AML cells. a RT-qPCR was used to detect the expression level of miR‐497‐5p in the bone marrow cells (BMCs) of AML patients (n = 30) and BMCs of healthy controls (n = 30). b RT-qPCR was used to detect the expression level of miR‐497‐5p AML cell lines (HL-60 and THP-1) and normal bone marrow cell line (HS-5). c HL-60 and THP-1 cells were treated with various concentrations of Tan IIA for 24 h. The expression level of miR‐497‐5p was examined by RT‐qPCR. **P < 0.01, ***P < 0.01 compared to DMSO control. d HL-60 cells were transfected with miR‐497‐5p mimic, miR‐497‐5p inhibitor, or its negative control. The expression level of miR‐497‐5p was examined by RT‐qPCR. e HL-60 cells were treated with (d), cell proliferation was examined by CCK‐8 assay. f HL-60 cells were treated with (d), the DNA synthesis ability of AML cells was detected by a bromodeoxyuridine incorporation assay. g HL-60 cells were treated as (d), cell apoptosis was examined by Annexin V and PI staining