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Fig. 3 | Cancer Cell International

Fig. 3

From: Tanshinone IIA regulates human AML cell proliferation, cell cycle, and apoptosis through miR-497-5p/AKT3 axis

Fig. 3

miR-497-5p directly targets the 3′UTR of AKT3. a The public miRNA databases, miRDB was scanned to predict targets of miR-497-5p in human. b HL-60 cells were co-transfected with miR-497-5p mimic or inhibitor and luciferase reporter plasmid containing wild-type or mutated miR-497-5p-binding site (mut) at AKT3 3′-UTR. Luciferase reporter assay was used to detect luciferase activity. c RT-qPCR was used to detect the expression level of AKT3 mRNA in the BMCs of AML patients and in the BMCs of healthy controls. d Spearman’s correlation analysis was used to examine the correlation between miR-497-5p and AKT3 mRNA levels in patients with AML. r = − 0.6057, P = 0.0004. e HL-60 cells were transfected with miR-497-5p mimic or inhibitor, respectively. The expression level of AKT3 in was detected by RT-qPCR. f HL-60 cells were transfected with miR-497-5p mimic and then treated with Tan IIA or not. The protein expression level of AKT3 was detected by western blot analysis. g HL-60 cells were transfected with miR-497-5p inhibitor and then treated with Tan IIA or not. The protein expression level of AKT3 was examined by western blot analysis

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