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Fig. 5 | Cancer Cell International

Fig. 5

From: A novel mechanism by which ACTA2-AS1 promotes cervical cancer progression: acting as a ceRNA of miR-143-3p to regulate SMAD3 expression

Fig. 5

ACTA2-AS1 modulated the expression of SMAD3 by repressing miR-143-3p. a The mRNA levels of SMAD3 in CC tissues were tested using qRT-PCR. b The mRNA expression of SMAD3 in cervical cancer cell lines was examined. c, d The mRNA and protein expression levels of SMAD3 were determined after transfecting miR‐143‐3p mimic and inhibitor. e, f. The mRNA and protein expressions of SMAD3 were tested after silencing ACTA2‐AS1. g The mRNA expression of SMAD3 was detected after transfected with si-NC, si-ACTA2-AS1, si-ACTA2-AS1 + miR-143-3p inhibitor and si-ACTA2-AS1 + inhibitor NC, respectively. GAPDH was used as an internal control. *P < 0.05; ** P < 0.01

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Cancer Cell International

ISSN: 1475-2867

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