Fig. 4

BRD4 is validated as a target of miR-338-3p in MM cells. a The complementary sites between miR-338-3p and the 3′UTR of BRD4 were predicted by targetscan software. b, c The luciferase activity was measured in H929 and OPM2 cells transfected with miR-NC or miR-338-3p and BRD4 3′UTR WT or BRD4 3′UTR MUT. d The protein level of BRD4 in BM-derived plasma cells of MM patients and healthy volunteers was detected by Western blot assay. e The level of BRD4 in H929, OPM2 and nPCs cells was evaluated by Western blot assay. f, g The linear relationship between BRD4 and miR-338-3p or circ_0007841 was analyzed using Spearman’s coefficient. h The expression of BRD4 was detected in MM cells transfected with miR-NC or miR-338-3p by Western blot assay. i The protein level of BRD4 was detected in MM cells transfected with Vector, circ_0007841, circ_0007841 + miR-NC or circ_0007841 + miR-338-3p by Western blot assay. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001