Fig. 7

MSCs-generated exosomes accelerate the malignant potential of MM cells via circ_0007841. a, b The expression of circ_0007841 was detected in the MSCs and MSCs-generated exosomes from the adjacent tissues of MM and normal tissues by qRT-PCR. c Western blot assay was performed to detect the protein levels of exosome-related markers, including CD63 and CD81, in cell lysate and exosomes. d The model showed that MM cells were co-cultured with MSCs, and only exosomes could move from the lower chambers to the upper chambers. e–l MM cells transfected with si-NC or si-circ_0007841#1 were co-cultured with MSCs or not. e, f CCK8 assay was performed to assess the proliferation of MM cells. g The proliferation of MM cells was evaluated by colony formation assay. h The cell cycle of MM cells was detected through conducting flow cytometry. i, j The abilities of migration and invasion of MM cells were assessed by transwell assays. k The apoptosis of MM cells was examined through performing flow cytometry. l The levels of p-PI3K, PI3K, p-AKT and AKT were detected in MM cells by Western blot assay. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001