Fig. 1
From: POLE2 knockdown reduce tumorigenesis in esophageal squamous cells
POLE2 is highly expressed in ESCC tissues and the construction of POLE2 knockdown cell model. a Expression levels of POLE2 in ESCC tumor tissues and normal skin tissues were detected by IHC staining. b POLE2 expression and overall survival of ESCC by Kaplan–Meier survival analysis. c Transfection efficiency for Eca-109 and TE-1 cells was evaluated by expression of green fluorescent protein 72 h post-infection. d, e The specificity and validity of the lentivirus-mediated shRNA knockdown of POLE2 expression was verified by qPCR (d) and western blot analysis (e). The data were presented as the mean ± SD (n = 3), *P < 0.05, **P < 0.01, ***P < 0.001