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Fig. 5 | Cancer Cell International

Fig. 5

From: LncRNA KCNQ1OT1 sponges miR-15a to promote immune evasion and malignant progression of prostate cancer via up-regulating PD-L1

Fig. 5

KCNQ1OT1 negatively regulated the expression of miR-15a and inhibited the cytotoxicity of CD8+ T cells. a, b The expression levels of KCNQ1OT1 (a) and miR-15a (b) were examined by RT-qPCR. DU145 or PC-3 cells were transfected pcDNA3.1-NC, pcDNA3.1-KCNQ1OT1, shNC, or shKCNQ1OT1. Non-transfected cells were used as control. c Bioinformatic analysis using Starbase (http://starbase.sysu.edu.cn/index.php) revealed potential binding sites between KCNQ1OT1 and miR-15a. d The relative luciferase activities were measured and compared between cells co-transfected with miR-15a mimics or miR-15a inhibitor. DU145 or PC-3 cells were transfected with luciferase reporter gene driven by either the wild type of KCNQ1OT1 (WT-KCNQ1OT1) or the mutated KCNQ1OT1 (MUT-KCNQ1OT1). e The protein level of PD-L1 was measured in indicated cells using western blot. f–h The cytotoxicity (f), proliferation (g), and apoptosis (h) of CD8+ T cells upon co-cultured with indicated PC cells were measured as described in Fig. 3. DU145 and PC-3 cells were not transfected or transfected with shNC, shKCNQ1OT1, shKCNQ1OT1 + inhibitor NC, or shKCNQ1OT1 + miR-15a inhibitor, respectively. *P < 0.05, **P < 0.01 and ***P < 0.001

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