Fig. 4

LINC00261 promoted DKK3 expression by recruiting GATA6. a The expression and location of LINC00261 measured by RNA-FISH (×400). b The binding capacity of GATA6 to the DKK3 protein detected by RIP, * p < 0.05 vs. IgG, **p < 0.01 vs. IgG. c The expression of DKK3 in all cell lines assessed by Western blot analysis. d DKK3 mRNA expression after overexpression or silencing of LINC00261 measured by RT-qPCR, *p < 0.05 vs. oe-NC, ^#p < 0.05 vs. si-NC; e The expression of DKK3 protein after overexpression or silencing of LINC00261 measured by Western blot analysis, *p < 0.05 vs. oe-NC, **p < 0.01 vs. oe-NC, ^#p < 0.05 vs. si-NC, ^##p < 0.01 vs. si-NC. f Two sites of GATA6 protein and DKK3 DNA identified by website analysis. g, h The fluorescence intensity in GATA6 and DKK3 promoter detected by dual luciferase reporter gene assay, *p < 0.05 vs. the NC, **p < 0.01 vs. the NC. i ChIP assay results; Statistical data are measurement data, and described as mean ± standard deviation, *p < 0.05 vs. IgG, **p < 0.01 vs. IgG. Unpaired t test is used to compare data between two groups. The experiment was repeated three times