Fig. 5

LINC00261 promoted the expression of DKK3 to suppress the metastasis and angiogenesis of prostate cancer by recruiting the transcription factor GATA6. a The expression of DKK3 and GATA6 assessed by RT-qPCR. b Representative images and statistical analysis of cell proliferation detected by EdU (×200). c, d Representative images and statistical analysis of cell migration (×200) and invasion (×200) detected by Transwell assay. e Representative images and statistical analysis of tube formation (×100). f, g The protein expression of MMP-2, MMP-9, VEGF and CD31 measured by Western blot analysis. *p < 0.05 vs. oe-NC group, **p < 0.01 vs. oe-NC group, ^#p < 0.05 vs. the cells treated oe-LINC00261 and si-NC, ^##p < 0.01 vs. the cells treated oe-LINC00261 and si-NC. Statistical data are measurement data and described as mean ± standard deviation. Unpaired t test is used to compare data between two groups. ANOVA is used to compare data among multiple groups, followed by Tukey’s post hoc test. The experiment was repeated three times