Skip to main content
Fig. 4 | Cancer Cell International

Fig. 4

From: Downregulation of long non-coding RNA MAFG-AS1 represses tumorigenesis of colorectal cancer cells through the microRNA-149-3p-dependent inhibition of HOXB8

Fig. 4

LncRNA MAFG-AS1 regulates the expression HOXB8 by targeting miR-149-3p. a The binding sites between miR-149-3p and HOXB8 predicted by Targetscan, which is an online prediction tool for biological targets of miRNA by identifying conservative 8mer, 7mer and 6mer sites that match the seed region of each miRNA. b The binding between miR-149-3p and HOXB8 detected by dual luciferase reporter gene assay. c The mRNA expression of HOXB8 in response to different expression patterns of miR-149-3p in HCT116 and LoVo cells determined by RT-qPCR. d The protein expression of HOXB8 in response to different expression patterns of miR-149-3p normalized to GAPDH in HCT116 and LoVo cells determined by Western blot analysis. e The mRNA expression of HOXB8 in response to different expression patterns of lncRNA MAFG-AS1 and miR-149-3p in HCT116 and LoVo cells determined by RT-qPCR. f The protein expression of HOXB8 in response to different expression patterns of lncRNA MAFG-AS1 and miR-149-3p normalized to GAPDH in HCT116 and LoVo cells determined by Western blot analysis. g The expression of HOXB8 in CRC tissues and adjacent tissues determined by RT-qPCR (n = 30). h The expression of HOXB8 in CRC tissues and adjacent tissues detected by immunohistochemistry (scale bar = 25 µm). Data between two groups were compared by unpaired t test but by paired t test between CRC tissues and adjacent tissues. Data among multiple groups were compared by one-way ANOVA, followed by Tukey’s post hoc test. The experiment was repeated 3 times independently

Back to article page