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Fig. 5 | Cancer Cell International

Fig. 5

From: Downregulation of long non-coding RNA MAFG-AS1 represses tumorigenesis of colorectal cancer cells through the microRNA-149-3p-dependent inhibition of HOXB8

Fig. 5

The lncRNA MAFG-AS1/miR-149-3p/HOXB8 axis promotes proliferation, migration and invasion of CRC cells in vitro. a The mRNA expression of HOXB8 in HCT116 and LoVo cells determined by RT-qPCR. b The protein expression of HOXB8 in HCT116 and LoVo cells normalized to GAPDH determined by Western blot analysis. c The expression of lncRNA MAFG-AS1, miR-149-3p and HOXB8 in HCT116 and LoVo cells determined by RT-qPCR. d The HCT116 and LoVo cell proliferation detected by CCK-8 assay. e The HCT116 and LoVo cell migration detected by Transwell assay (scale bar = 50 µm). f The HCT116 and LoVo cell invasion detected by Transwell assay (scale bar = 50 µm). g The HCT116 and LoVo cell apoptosis detected by flow cytometry. Data among multiple groups were compared by one-way ANOVA, followed by Tukey’s post hoc test. Data at different time points were analyzed by repeated measures ANOVA, followed by Bonferroni test. The experiment was repeated 3 times independently

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Cancer Cell International

ISSN: 1475-2867

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