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Fig. 4 | Cancer Cell International

Fig. 4

From: EIF3J-AS1 promotes glioma cell growth via up-regulating ANXA11 through sponging miR-1343-3p

Fig. 4

ANXA11 is the downstream target of miR-1343-3p. a The expression of ANXA11, the target of miR-1343-3p, was evaluated in glioma cell lines and normal glial HEB. b, c The expression of ANXA11 was detected by qRT-PCR after transfecting miR-1343-3p mimics or miR-1343-3p inhibitor. d, e The expression levels of ANXA11 were detected in response to sh-ANXA11 and pcDNA3.1/ANXA11. f RIP assay showed co-precipitated EIF3J-AS1, miR-1343-3p and ANXA11 by antibody targeting Ago2. g RNA pull down validated the interaction between ANXA11 and miR-1343-3p. h Putative and mutant binding sequences of miR-1343-3p in 3′UTR sequence of ANXA11. i Dual-luciferase reporters showed that miR-1343-3p mimics could impair the luciferase activity of ANXA11-WT. *P < 0.05, **P < 0.01

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