Skip to main content
Fig. 2 | Cancer Cell International

Fig. 2

From: p33ING1b regulates acetylation of p53 in oral squamous cell carcinoma via SIR2

Fig. 2

Over expression of ING1b increase acetylated p53, decrease cell proliferation, and induce apoptosis. a Immunoblot analysis of ING1b, total and acetylated p53 (at Lys382), and pro-apoptotic Bax and p21 in YD-9 and YD-8 cells transiently transfected with control or ING1 expression plasmid. GAPDH was used as a loading control. Data is representative of three independent experiments. Numbers below the figure shows relative expression of ING1b as determined by densitometry analysis. b Cell proliferation was assayed for 3 days in YD-9 and YD-8 cells transiently transfected with control or ING1 expression plasmid, 48 h after transfection. Data represented in from 3 different independent experiments. Error bars, SD. c Immunoblot analysis of anti-apoptotic Bcl-XL and pro-apoptotic Cleaved Caspase-3 in YD-8 and YD-9 cells transiently transfected with control or ING1b expression plasmids. GAPDH was used as a loading control. Data is representative of three independent experiments. d Immunoblot analysis of ING1b, total and acetylated p53 (at Lys382), anti-apoptotic Bcl-XL and pro-apoptotic Cleaved Caspase-3 in Ca9-22 and Sa-3 cell lines transiently transfected with control or ING1b expression plasmids. GAPDH was used as a loading control. Data is representative of three independent experiments

Back to article page