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Fig. 3 | Cancer Cell International

Fig. 3

From: Circ_002117 binds to microRNA-370 and promotes endoplasmic reticulum stress-induced apoptosis in gastric cancer

Fig. 3

Ectopic expression of circ_002117 facilitates ER stress-induced apoptosis in gastric cancer cells. a Structure of circ_002117. b RT-qPCR determination of circ_002117 expression in AGS cells after overexpression of circ_002117. c linear RNA-002117 expression in AGS cells after overexpression of circ_002117 determined by RT-qPCR. d EdU assay determining in AGS cell proliferation after overexpression of circ_002117 (×200) and quantitation of the proliferation rate. e Apoptosis in AGS cells after overexpression of circ_002117 determined by flow cytometry and quantitation of the apoptotic rate. f Ultrastructure of AGS cells after overexpression of circ_002117 observed by transmission electron microscope (×10,000). Arrow refers to ER. g Immunofluorescence staining for detection of GRP78 after overexpression of circ_002117 (×200). h Western blot analysis was performed to detect expression of ER stress marker, including GRP78, IRE1, CHOP and Eif2α, marker of ER stress-induced apoptosis (caspase 12) and its downstream apoptotic factor (cleaved-caspase3). i Representative macroscopic image of a xenograft tumor after treatment with oe-circ_002117 or oe-NC. j Quantitation of tumor volume (N = 05) upon treatment with oe-circ_002117 or oe-NC. k Quantitation of tumor volume (N = 05) upon treatment with oe-circ_002117 or oe-NC. l Immunohistochemistry of cleaved-capase3 expression in xenograft tumors of mice treated with oe-circ_002117 or oe-NC (×400). *p < 0.05 vs.. AGS cells or mice treated with oe-NC. Data are shown as the mean ± standard deviation. Data between two groups were analyzed unpaired t-test. Variables were analyzed at different time points using Bonferroni-corrected repeated measures ANOVA. The cell experiment was repeated in triplicate

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