Fig. 7

Circ_002117 facilitates ER stress-induced apoptosis in gastric cancer by targeting miR-370-HERPUD1 axis. a Correlation between circ_002117 and HERPUD1 in tissues. b HERPUD1 expression after oe-circ_002117 treatment determined by RT-qPCR. c Proliferation in AGS cells after alteration of circ_002117, miR-370 and HERPUD1 determined by EdU (×200). d Quantification of proliferation rate. e Apoptosis in AGS cells after alteration of circ_002117, miR-370 and HERPUD1 determined by flow cytometry and quantification of apoptotic rate. f Ultrastructure of AGS cells after alteration of circ_002117, miR-370 and HERPUD1 observed by the transmission electron microscopy (×10,000). Arrow refers to ER. g GPR78 expression in AGS cells after alteration of circ_002117, miR-370 and HERPUD1 determined by immunofluorescence staining (×200). h Related protein expression in AGS cells after alteration of circ_002117, miR-370 and HERPUD1 determined by western blot. i Corresponding quantitation concerning h. *p < 0.05 vs. oe-NC. ^#p < 0.05 vs. oe-circ_002117 + mimic NC. Data were shown as the mean ± standard deviation. Statistical comparisons were performed by Tukey’s test-corrected one-way ANOVA when more than two groups were compared. The experiment was repeated in triplicate