Fig. 2

TW-37 induces apoptosis in human oral cancer cell lines by suppressing the Mcl-1 expression. a The expressions of myeloid cell leukemia-1 (Mcl-1), Bcl-2, and Bcl-xL were detected using Western blotting. Actin was used as a loading control. b The expression levels of Mcl-1 and cleaved PARP were detected using Western blotting. Actin was used as a loading control. c Relative mRNA levels of Mcl-1 were measured by qPCR and normalized to GAPDH. All bar graphs represent mean ± SD of three independent experiments. *P < 0.05. d HSC-3 cell lines were pretreated with CHX for 1 h, followed by treatment with 5-μM TW-37 for 48 h. The expression of Mcl-1 was analyzed using Western blotting. e After treatment with MG132 for 1 h, the HSC-3 cell lines were treated with 5-μM TW-37 for the indicated time points. The protein levels of Mcl-1 were detected with Western blotting. Actin was used as a loading control. Data represent the mean of the two independent experiments