Fig. 4

Effect of miR-326 down-regulation on silence of circ_0000517-mediated hepatocellular carcinoma progression. a miR-326 abundance was examined in HCCLM3 and Huh7 cells transfected with anti-miR-326 or anti-NC. Cell viability (b, c), colony formation (d), Ki67 and PCNA protein levels (e), migration (f), invasion (g), E-cadherin and Vimentin protein levels (h), glucose consumption (i), lactate production (j), ATP production (k), and LDHA and HK2 protein expression (l) were measured in HCCLM3 and Huh7 cells transfected with si-NC, si-circ_0000517#3, si-circ_0000517#3 + anti-miR-326 or anti-NC. *P < 0.05