Fig. 5

The interaction between miR-326 and IGF1R. a The effect of miR-326 on the 10 targets levels was measured in HCCLM3 cells. b The binding sequence of miR-326 and IGF1R. (c, d) Luciferase activity was examined in HCCLM3 and Huh7 cells transfected with IGF1R 3′UTR-WT or IGF1R 3′UTR-MUT and miR-326 mimic or miR-NC. e, f IGF1R expression was measured in HCCLM3 and Huh7 cells with transfection of miR-326 mimic or miR-NC. g, h IGF1R expression was measured in tumor and adjacent nontumor tissues from patients with hepatocellular carcinoma. n = 3. i, j IGF1R level was examined in HCCLM3, Huh7 and THLE-2 cells. k The relationship between IGF1R and miR-326 or circ_0000517 levels in hepatocellular carcinoma tissues. ^*P < 0.05