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Fig. 4 | Cancer Cell International

Fig. 4

From: Circ_0000140 restrains the proliferation, metastasis and glycolysis metabolism of oral squamous cell carcinoma through upregulating CDC73 via sponging miR-182-5p

Fig. 4

Circ_0000140 could absorb miR-182-5p. a The sequences of circ_0000140 containing the miR-182-5p binding sites or mutant binding sites were presented. b, c Dual-luciferase reporter assay was used to detect the interaction between circ_0000140 and miR-182-5p in CAL-27 and SCC-4 cell. d, e The enrichment of circ_0000140 and miR-182-5p in anti-Ago2 or anti-IgG was measured by the RIP assay. f qRT-PCR was performed to measure the expression of miR-182-5p in OSCC cells (CAL-27 and SCC-4) and NOK cells. g The expression of miR-182-5p in CAL-27 and SCC-43 cells was assessed by qRT-PCR to assess the effect of circ_0000140 overexpression on miR-182-5p expression. * P < 0.05, ** P < 0.01

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Cancer Cell International

ISSN: 1475-2867

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