Fig. 5

Effects of miR-182-5p mimic on the progression of OSCC cells. a The expression of miR-182-5p in CAL-27 and SCC-4 cells was measured by qRT-PCR to evaluate the transfection efficiency of miR-182-5p mimic. CAL-27 and SCC-4 cells were co-transfected with circ_0000140 overexpression vector and miR-182-5p mimic. b The number of colonies in CAL-27 and SCC-4 cells was detected by colony formation assay. c, d The number of migrated and invaded CAL-27 and SCC-4 cells was tested by transwell assay. e, f The protein levels of ki67, MMP-2 and MMP-9 in CAL-27 and SCC-4 cells were assessed by WB analysis. g Seahorse XF Extracellular Flux Analyzer was used to evaluate the ECAR of CAL-27 and SCC-4 cells. h The lactate acid level of CAL-27 and SCC-4 cells was determined by Lactate Assay Kit. i, j WB analysis was performed to measure the protein levels of GLUT1 and LDHA in CAL-27 and SCC-4 cells. ** P < 0.01