Fig. 7

Effects of CDC73 silencing on the progression of OSCC cells. a The protein level of CDC73 in CAL-27 and SCC-4 cells was measured by WB analysis to evaluate the transfection efficiency of si-CDC73. CAL-27 and SCC-4 cells were co-transfected with anti-miR-182-5p and si-CDC73. b Colony formation assay was performed to assess the number of colonies in CAL-27 and SCC-4 cells. c The number of migrated and invaded CAL-27 and SCC-4 cells was detected by transwell assay. d WB analysis was used to measure the protein levels of ki67, MMP-2 and MMP-9 in CAL-27 and SCC-4 cells. e, f Seahorse XF Extracellular Flux Analyzer was employed to assess the ECAR of CAL-27 and SCC-4 cells. g The lactate acid level of CAL-27 and SCC-4 cells was detected by Lactate Assay Kit. h The protein levels of GLUT1 and LDHA in CAL-27 and SCC-4 cells were tested by WB analysis. ** P < 0.01