Fig. 4From: Development of syngeneic murine cell lines for use in immunocompetent orthotopic lung cancer modelsValidation of a KrasG12D.Map3k7−/−.GFP+ cell line. a H&E stain showing orthotopic tumor formation by line E889. Scale bar is 100 µm and the red line denotes the tumor border. b PCR of genomic DNA showing recombination of the KrasG12D allele (305 bp) and Map3k7 allele (1.2 kb) in E889 cells but not in CMT or LLC controls. c Western blot showing expression of KRASG12D and loss of MAP3K7 in E889 cells. d GFP expression in E899 cells. Cells were heat fixed to a glass slide, counterstained with DAPI, and imaged by fluorescent microscopy; scale bar is 50 µmBack to article page