Table 1 PCR primers used to validate cell lines
From: Development of syngeneic murine cell lines for use in immunocompetent orthotopic lung cancer models
| Target | Recombinant PCR-forward | Recombinant PCR-reverse | Recombinant PCR-reverse 2 |
|---|---|---|---|
| p53 | CACAAAAACAGGTTAAACCCA | GAAGACAGAAAAGGGGAGGG | |
| Kras | GGGTAGGTGTTGGGATAGCTG | TCCGAATTCAGTGACTACAGATGTACAGAG | |
| PTEN | ACTCAAGGCAGGGATGAGC | AATCTAGGGCCTCTTGTGCC | GCTTGATATCGAATTCCTGCAGC |
| SMAD4 | TCCCACATTCCTCTTAGTTTTGA | CCAGCTTCTCTGTCCAGGTAGTA | |
| PIK3CA | CACAGCTCGCGGTTGAGG | TGCTCGACGTTGTCACTGAA | CGGGTGTACTCCTCATATAACA |
| TGFβR2 | AGGGATGAATGGGCTTGCTT | CTCACCTCAGAGCCTGATTA | |
| TAK1 | GCAACTTCGACAACTTGCCTTCCTGTG | GCACTTGAATTAGCGGCCGCAAGCTTATAACT | |
| EML-ALK | GAGCCTTGTTGATACATCGTTC | TAGGAGGCAGTTTGGGCTAC | CAAGGCAGTGAGAACCTGAA |
