Fig. 1
Methylation of BM742401 in healthy controls and HMCLs. a Direct sequencing of M-MSP products from positive control with methylated DNA showed the conversion of all unmethylated cytosines into uracils (turned into thymidines after PCR) but all methylated cytosines remained unchanged, indicating complete bisulfite conversion and specificity of MSP. b M-MSP and U-MSP showed that all healthy controls (N1-N17) were completely unmethylated (UU), whereas positive control with methylated DNA was completely methylated (MM). c M-MSP and U-MSP showed BM742401 was MM in HMCLs, including KMS-12-PE, MOLP-8 and OCI-MY5, partially methylated (MU) in JJN-3, LP-1, OPM-2, U-266, WL-2, OPM-2/BTZ and RPMI-8226R, UU in NCI-H929, RPMI-8226, MMKKF, MMLAL and KMS-11/BTZ