Fig. 2

Methylation and expression of BM742401 in HMCLs. a By qRT-PCR, methylation of BM742401 was significantly correlated with lower expression level and hence larger ΔCt of BM742401 (MM vs. UU, P = 0.041; MM + MU vs. UU, P = 0.047). b In MOLP-8 cells, which were completely methylated for BM742401, treatment with 5-AzadC for 5 days led to BM742401 promoter demethylation, as evidenced by emergence of U-MSP signal (upper), and concomitant re-expression of BM742401 (lower). Error bars represent standard deviation from three independent qRT-PCR