Fig. 2

NNT-AS1 served as miR-496 sponge in PCa cells. a Subcellular fractionation assay demonstrated the location of NNT-AS1 in PCa cells. b FISH experiments further proved the distribution of NNT-AS1 in PCa cells. c MS2-RIP assays were conducted in both VCaP and PC3 cells to assess the binding of 9 miRNA candidates toNNT-AS1. d RT-qPCR detected the expression of miR-496 in PCa cells. e ENCORI revealed the binding sites between NNT-AS1 and miR-496. f RNA pull down assay was performed to attest the enrichment of NNT-AS1 in indicated groups. g Luciferase reporter assay further validated the interaction between NNT-AS1 and miR-496 in VCaP and PC3 cells. *p < 0.05, **p < 0.01