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Fig. 4 | Cancer Cell International

Fig. 4

From: NNT-AS1 modulates prostate cancer cell proliferation, apoptosis and migration through miR-496/DDIT4 axis

Fig. 4

NNT-AS1 promoted the malignant behaviors in PCa cells through targeting miR-496/DDIT4 pathway. a The expression of DDIT4 was detected using RT-qPCR in VCaP and PC3 cells under NNT-AS1 silence or miR-496 enhancement. b, c RT-qPCR examined miR-496 expression in PCa cells after transfection with miR-496 inhibitor or NC inhibitor, and DDIT4 expression in cells with transfection of pcDNA3.1-DDIT4 or pcDNA3.1-NC. d CCK-8 assay examined the viability of VCaP and PC3 cells transfected with sh-NC, sh-NNT-AS1#1, sh-NNT-AS1#1 + miR-496 inhibitor, or sh-NNT-AS1#1 + pcDNA3.1-DDIT4. e, f EdU and colony formation assays measured the proliferation of VCaP and PC3 cells under above conditions. g Flow cytometry assay assessed the apoptosis rate in indicated VCaP and PC3 cells. h JC-1 assay detected cell apoptosis in indicated VCaP and PC3 cells. i Wound healing experiments demonstrated the migration ability of indicated VCaP and PC3 cells. **p < 0.01

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Cancer Cell International

ISSN: 1475-2867

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