Fig. 6
From: MicroRNA-214-3p inhibits the stem-like properties of lung squamous cell cancer by targeting YAP1
Rescue experiments confirm that YAP1 liberated the growth and CSC properties repressed by miR-214. miR-214-3p represses growth and CSC properties by inhibiting YAP1. a CCK experiments in LSCC cells transfected with different plasmids. b Colony formation in cells transduced with different plasmids. c Sphere-forming experiments in LSCC cells transfected with different plasmids. d Re-introduction of YAP1 rescued the reductions of CSC-specific markers in both cell lines. d, e. Vimentin and Snail protein levels were detected in LSCC cells transfected with different plasmids. e, f. In subcutaneous mice models, the re-expression of YAP1 remarkably decreased the anti-tumor effects of miR-214-3p