Fig. 2

Knockdown of MIR99AHG suppressed GC cell proliferation, invasion and migration in vitro. a MIR99AHG expression was measured by qPCR. b, c Growth curves were measured by CCK-8 assay. d Trans-well assay; upper panel shows migration effect and lower panel shows invasion effect. e, f Qualification of the trans-well assay. g E-cadherin and N-cadherin expression levels were detected by Western blotting. h, i Knockdown MIR99AHG promoted apoptosis in GC cells. Representative FACS images and statistics based on three independent experiments. j Apoptosis related key proteins were detected by Western blotting. *p < 0.05, **p < 0.01 and ***p < 0.001 (two-way ANOVA)