Fig. 2

A combination of NDV and MH showed superior anticancer activity in comparison to monotherapies in both AMJ13 and MCF-7 breast cancer cells. However, there was no enhanced toxicity against non-cancerous REF cells. (a, c and e) AMJ13, MCF-7, and REF cells were treated with NDV (0.3, 1, and 2 MOI) and with MH (62.5, 125, and 250 μg/ml), then cell viability was measured by MTT assay. d–f Illustrations of normalized isobologram of nonconstant combination ratios were measured by the Chou-Talalay method, where CI value quantitatively defines synergism. (CI < 0.9), additive effect (CI = 0.9–1.1) and antagonism (CI > 1.1). All data shown are mean ± SEM (*P < 0.05 compared to mono-treatments) data from three different experiments