Fig. 3

Identification of RHBDD1 as a direct target gene of miR-924 in NSCLC cells. a Bioinformatics prediction revealed a highly conserved miR-924 binding site in the 3′-UTR of RHBDD1. b, c H1299 or A549 cells were transfected with either miR-924 mimic or miR-NC and either wild-type (WT) or mutant (MUT) 3′-UTR RHBDD1 reporter plasmid. Luciferase activity was measured at 48 h post-transfection and normalized to that of the Renilla luciferase activity. d, e Expression levels of RHBDD1 mRNA and protein in miR-924-overexpressing H1299 and A549 cells were measured by using quantitative real time PCR and western blot analysis, respectively. **p < 0.01, ***p < 0.001, compared with miR-NC