Fig. 1

CircRAE1 was upregulated in CRC. a Expression of CircRAE1 in paired CRC tissues from 80 CRC patients measured by qRT-PCR. b Relative expression levels of CircRAE1 in normal cell line (NM460) and CRC cell lines (HCT116, SW620, HT29, and SW480) by qRT-PCR. c Relative expression level of CircRAE1 and its linear types in SW620 and HT29 cells at various time points after treatment by actinomycin D. d Total RNA from SW620 or HT29 cells treated with RNase R. CircRAE1 was stably expressed in both RNase R(−) and RNase R(+) total RNA samples. e RNA in situ hybridization for CircRAE1 (red) in sw620 and HT29 cells (× 200). Nuclei were stained with DAPI (blue). Scare bar = 100 μm.*P < 0.05, **P < 0.01, ***P < 0.001