Fig. 3

CircRAE1 serves as ceRNA for miR-338-3p in CRC cells. a Expression of miR-338-3P in paired CRC tissues from 80 CRC patients as measured by qRT-PCR. b CircRAE1 expression that is negatively correlated with miR-338-3P expression in CRC tissues. c Downregulation of CircRAE1-promoted miR-338-3p expression in SW620 and HT29 cells as determined by qRT-PCR. d RNA in situ hybridization for miR-338-3p (green) and CircRAE1 (red) in SW620 cells (× 200). Nuclei were stained with DAPI (blue). Scare bar = 100 μm e Significantly reduced luciferase activity of CircRAE1 WT by miR-338-3p mimics. *P < 0.05, **P < 0.01, ***P < 0.001