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Fig. 1 | Cancer Cell International

Fig. 1

From: NONO promotes hepatocellular carcinoma progression by enhancing fatty acids biosynthesis through interacting with ACLY mRNA

Fig. 1

NONO promotes DEN-induced HCC cell growth and invasion, and is associated with FA synthesis signaling. a NONO protein expression levels in DEN-stimulated SMMC-7721 or MHCC97H cells were detected by Western blotting. b NONO mRNA expression levels in DEN-stimulated SMMC-7721 or MHCC97H cells were detected by qRT-PCR. c, d SMMC-7721 or MHCC97H cells were transfected with si-NONO for 24 h, and then treated with DEN for 24 h. Cell invasive ability was examined by transwell invasion assays (c). Cell proliferation was examined by MTT assays (d). e Summary of NONO-associated RNAs in DEN-stimulated MHCC97H cells, determined by RIP-seq by precipitation with NONO antibody. f Annotation of the NONO RIP-seq peaks. g Pathway enrichment analysis of the overlapped 222 genes identified in MHCC97H cells stimulated with DEN for 12 and 24 h. h Heatmap analysis of NONO binding genes involved FA biosynthesis. ATP-citrate lyase, ACLY; Short chain enoyl-CoA hydratase 1, ECHS1; Ayl-CoA dehydrogenase very long chain, ACADVL; Acyl-CoA dehydrogenase short chain, ACADS; Stearoyl-CoA-desaturase 1, SCD1; Fatty acid synthase, FASN; Acetyl-CoA carboxylase, ACC. Data are represented as mean ± SD (n = 3; *p < 0.05)

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