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Fig. 2 | Cancer Cell International

Fig. 2

From: NONO promotes hepatocellular carcinoma progression by enhancing fatty acids biosynthesis through interacting with ACLY mRNA

Fig. 2

NONO promotes HCC progression by interacting with ACLY mRNA to enhance FA biosynthesis. a RIP analysis of the interactions between NONO and ACLY mRNA in DEN-stimulated SMMC-7721 or MHCC97H cells. b RIP analysis of the interactions between NONO and ACLY mRNA in MHCC97H cells transfected with si-NONO for 24 h, and then stimulated with DEN for 24 h. c Western blotting analysis of the NONO and ACLY protein expressions in MHCC97H cells transfected with si-NONO for 24 h, and then stimulated with DEN for 24 h. d qRT-PCR analysis of ACLY mRNA expression in MHCC97H cells transfected with si-NONO for 24 h, and then stimulated with DEN for 24 h. e CHIP analysis of the RNA polymerase II (Pol II) binding on ACLY promoter region in MHCC97H cells transfected with si-NONO for 24 h, and then stimulated with DEN for 24 h. f qRT-PCR analysis of the distribution ratio of ACLY mRNA in MHCC97H cells transfected with si-NONO for 24 h, and then stimulated with DEN for 24 h. g Citrate and oxaloacetate productions were determined in MHCC97H cells transfected with si-NONO for 24 h, and then stimulated with DEN for 24 h. h Metabolomics analysis of unsaturated long-chain or polyunsaturated fatty acids in MHCC97H cells transfected with si-NONO for 24 h, and then stimulated with DEN for 24 h. i, j MHCC97H cells were transfected with si-NONO or/and pCMV-Myc-NONO vector for 24 h, and then treated with DEN for 24 h. Cell invasive ability was examined by transwell invasion assays (i). Cell proliferation was examined by MTT assays (j). Data are represented as mean ± SD (n = 3; *p < 0.05)

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