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Fig. 3 | Cancer Cell International

Fig. 3

From: NONO promotes hepatocellular carcinoma progression by enhancing fatty acids biosynthesis through interacting with ACLY mRNA

Fig. 3

NONO promoting ACLY expression is not related to nuclear paraspeckles in HCC cells. a NEAT1_2 expressions in DEN-stimulated MHCC97H cells were detected by qRT-PCR. b RIP analysis of the interactions between NONO and NEAT1_2 in DEN-stimulated MHCC97H cells. c Immunofluorescence analysis of NONO and NEAT1_2 distributions in DEN-stimulated MHCC97H cells (NONO, green; NEAT1_2, representative of Paraspeckle, red; Nucleus, blue). d qRT-PCR analysis of ACLY mRNA expression in MHCC97H cells transfected with si-NEAT1_2 for 24 h, and then stimulated with DEN for 24 h. e RIP analysis of the interactions between NONO and ACLY mRNA in MHCC97H cells transfected with si-NEAT1_2 for 24 h, and then stimulated with DEN for 24 h. Data are represented as mean ± SD (n = 3; *p < 0.05)

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Cancer Cell International

ISSN: 1475-2867

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