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Fig. 4 | Cancer Cell International

Fig. 4

From: NONO promotes hepatocellular carcinoma progression by enhancing fatty acids biosynthesis through interacting with ACLY mRNA

Fig. 4

NONO promotes nuclear ACLY mRNA stability, and IGF2BP1 increases cytoplastic ACLY mRNA stability in DEN stimulated HCC cells. ac qRT-PCR analysis of whole ACLY mRNA expression (a), nuclear ACLY mRNA expression (b), or cytoplasmic ACLY mRNA expression (c) in MHCC97H cells transfected with si-NONO for 24 h, stimulated with DEN for 24 h, then treated with the transcriptional inhibitor Actinomycin D (ActD, 10 μg/ml) for indicated hours. d Proteins interacted with NONO in MHCC97H cells identified with COIP. e COIP analysis of the interactions of NONO and IGF2BP1 in DEN-stimulated MHCC97H cells. f RIP analysis of the interactions between IGF2BP1 and ACLY mRNA in DEN-stimulated MHCC97H cells. g RIP analysis of the interactions between NONO/IGF2BP1 and ACLY mRNA in the nuclear or cytoplasmic fractions of DEN-stimulated MHCC97H cells. h, i qRT-PCR analysis of nuclear ACLY mRNA expression (h), or cytoplasmic ACLY mRNA expression (i) in MHCC97H cells transfected with si-IGF2BP1 for 24 h, stimulated with DEN for 24 h, then treated with ActD for indicated hours. Data are represented as mean ± SD (n = 3; *p < 0.05)

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