Skip to main content
Fig. 6 | Cancer Cell International

Fig. 6

From: NONO promotes hepatocellular carcinoma progression by enhancing fatty acids biosynthesis through interacting with ACLY mRNA

Fig. 6

NONO/SFPQ heterodimer is essential for NONO interacting with ACLY mRNA in HCC cells. a Western blotting analysis of SFPQ expression in wild type MHCC97H cells (WT) or SFPQ-knockout MHCC97H cells (SFPQ-Cas9). b RIP analysis of the interactions between NONO and ACLY mRNA in wild type MHCC97H cells (WT) or SFPQ-knockout MHCC97H cells (SFPQ-Cas9). c RIP analysis of the interactions between NONO and ACLY mRNA in wild type MHCC97H cells (WT), SFPQ-knockout MHCC97H cells transfected with pCMV-FLAG vector or pCMV-FLAG-SFPQ. d Western blotting analysis of NONO expression in wild type MHCC97H cells (WT) or NONO-knockout MHCC97H cells (NONO-Cas9). e RIP analysis of the interactions between NONO and ACLY mRNA in wild type MHCC97H cells (WT) or NONO-knockout MHCC97H cells (NONO-Cas9). f RIP analysis of the interactions between NONO and ACLY mRNA in wild type MHCC97H cells (WT), NONO-knockout MHCC97H cells (NONO-Cas9) transfected with pCMV-MYC vector or pCMV-MYC-NONO. g RIP analysis of the interactions between NONO and ACLY mRNA in wild type MHCC97H cells (WT), NONO-knockout MHCC97H cells (NONO-Cas9) transfected with pCMV-MYC vector, pCMV-MYC-NONO ΔR1 + R2 or pCMV-MYC-NONO ΔR1. Data are represented as mean ± SD (n = 3; *p < 0.05)

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us