Name | Sequence | |
---|---|---|
Primers for NONO constructs | ||
NONO F | 5′-GCCATGGAGGCCCGAATTCGGATGCAGAGTAATAAAACTTTTAACT-3′ | pCMV-Myc-NONO |
NONO R | 5′-GGCCGCGGTACCTCGAGTTAGTATCGGCGACGTTTGTTTG-3′ | |
N terminal deletion of NONO (ΔN) F | 5′-GCCATGGAGGCCCGAATTCGGCGTCTTTTTGTGGGAAATCT-3′ | pCMV-Myc-NONO ΔN |
N terminal deletion of NONO (ΔN) R | 5′-GGCCGCGGTACCTCGAGTTAGTATCGGCGACGTTTGTTTG-3′ | |
C terminal deletion of NONO (ΔC) F | 5′-GCCATGGAGGCCCGAATTCGGATGCAGAGTAATAAAACTTTTAACT-3′ | pCMV-Myc-NONO ΔC |
C terminal deletion of NONO (ΔC) R | 5′-GGCCGCGGTACCTCGAGTTAGTATTCCCTTGAATCCTTCC-3′ | |
DHBS domain of NONO (DHBS) F | 5′-GCCATGGAGGCCCGAATTCGGCGTCTTTTTGTGGGAAATC-3′ | pCMV-Myc-NONO DHBS |
DHBS domain of NONO (DHBS) R | 5′-GGCCGCGGTACCTCGAGTTAGTATTCCCTTGAATCCTTCC-3′ | |
RRM1 deletion of NONO DHBS domain (DHBSΔR1) F | 5′-GCCATGGAGGCCCGAATTCGGTCCCTTACAGTTCGAAACCT-3′ | pCMV-Myc-NONO DHBSΔR1 |
RRM1 deletion of NONO DHBS domain (DHBSΔR1) R | 5′-GGCCGCGGTACCTCGAGTTAGTATTCCCTTGAATCCTTCC-3′ | |
Both RRM1 and RRM2 deletion of NONO DHBS domain (DHBSΔR1 + ΔR2) F | 5′-GCCATGGAGGCCCGAATTCGGTTAGATGATGAAGAGGGAC-3′ | pCMV-Myc-NONO DHBSΔR1 + ΔR2 |
Both RRM1 and RRM2 deletion of NONO DHBS domain (DHBSΔR1 + ΔR2) R | 5′-GGCCGCGGTACCTCGAGTTAGTATTCCCTTGAATCCTTCC-3′ | |
SFPQ F | 5′-CGGTCGACCATGTCTCGGGATCGGTTC-3′ | pCMV-Flag-SFPQ |
SFPQ R | 5′-CGGGGTACCCTAAAATCGGGGTTTTTT-3′ | |
Primers for qRT-PCR | ||
NONO F | 5′-CTAGCGGAGATTGCCAAAGTG-3′ | |
NONO R | 5′-GTTCGTTGGACACATACTGAGG-3′ | |
ACLY F | 5′-ATCGGTTCAAGTATGCTCGGG-3′ | |
ACLY R | 5′-GACCAAGTTTTCCACGACGTT-3′ | |
NEAT1_2 F | 5′-CTAGAGGCTCGCATTGTGTG-3′ | |
NEAT1_2 R | 5′-GCCCACACGAAACCTTACAT-3′ | |
GAPDH F | 5′-TCAACAGCAACTCCCACTCTTCCA-3′ | |
GAPDH R | 5′-ACCCTGTTGCTGTAGCCGTATTCA-3′ | |
The sequences of siRNAs | ||
si-NONO | 5′-CAGGCGAAGUCUUCAUUCA-3′ | |
si-ACLY | 5′-GAUCAAACGUCGUGGAAAAUU-3′ | |
si-NEAT1_2 | 5′-GGAGGAGUCAGGAGGAAUAUU-3′ | |
si-IGF2BP1 | 5′-CCUGGCUGCUGUAGGUCUU-3′ | |
Scrambled siRNA | 5′-UUCUCCGAACGUGUCACGUTT-3′ | |
Primers used for ChIP | ||
ACLY promoter (0–0.5 k) F | 5′-GCTGGGATTACAGGCATGAGCCA-3′ | |
ACLY promoter (0–0.5 k) R | 5′-GACTACAGGAGCATGCCACC-3′ | |
ACLY promoter (0.5–1 k) F | 5′-ATAAGATCTAGCCCCAGCTAAGTG-3′ | |
ACLY promoter (0.5–1 k) R | 5′-CAAGAATCGCTTGAACCCGG-3′ | |
ACLY promoter (1–1.5 k) F | 5′-GGCCCGAAGTCCACCGTGCCG-3′ | |
ACLY promoter (1–1.5 k) R | 5′-CGGACCTCACCAAGGCAGGC-3′ | |
ACLY promoter (1.5–2 k) F | 5′-CGGGTTCGGGCCCCGGCTCGG-3′ | |
ACLY promoter (1.5–2 k) R | 5′- CGGGGATCTCTGCAATGGA-3′ |