Skip to main content

Table 1 Primers used in this study (F, forward; R, reverse)

From: NONO promotes hepatocellular carcinoma progression by enhancing fatty acids biosynthesis through interacting with ACLY mRNA

Name Sequence  
Primers for NONO constructs
 NONO F 5′-GCCATGGAGGCCCGAATTCGGATGCAGAGTAATAAAACTTTTAACT-3′ pCMV-Myc-NONO
 NONO R 5′-GGCCGCGGTACCTCGAGTTAGTATCGGCGACGTTTGTTTG-3′
 N terminal deletion of NONO (ΔN) F 5′-GCCATGGAGGCCCGAATTCGGCGTCTTTTTGTGGGAAATCT-3′ pCMV-Myc-NONO ΔN
 N terminal deletion of NONO (ΔN) R 5′-GGCCGCGGTACCTCGAGTTAGTATCGGCGACGTTTGTTTG-3′
 C terminal deletion of NONO (ΔC) F 5′-GCCATGGAGGCCCGAATTCGGATGCAGAGTAATAAAACTTTTAACT-3′ pCMV-Myc-NONO ΔC
 C terminal deletion of NONO (ΔC) R 5′-GGCCGCGGTACCTCGAGTTAGTATTCCCTTGAATCCTTCC-3′
 DHBS domain of NONO (DHBS) F 5′-GCCATGGAGGCCCGAATTCGGCGTCTTTTTGTGGGAAATC-3′ pCMV-Myc-NONO DHBS
 DHBS domain of NONO (DHBS) R 5′-GGCCGCGGTACCTCGAGTTAGTATTCCCTTGAATCCTTCC-3′
 RRM1 deletion of NONO DHBS domain (DHBSΔR1) F 5′-GCCATGGAGGCCCGAATTCGGTCCCTTACAGTTCGAAACCT-3′ pCMV-Myc-NONO DHBSΔR1
 RRM1 deletion of NONO DHBS domain (DHBSΔR1) R 5′-GGCCGCGGTACCTCGAGTTAGTATTCCCTTGAATCCTTCC-3′
 Both RRM1 and RRM2 deletion of NONO DHBS domain (DHBSΔR1 + ΔR2) F 5′-GCCATGGAGGCCCGAATTCGGTTAGATGATGAAGAGGGAC-3′ pCMV-Myc-NONO DHBSΔR1 + ΔR2
 Both RRM1 and RRM2 deletion of NONO DHBS domain (DHBSΔR1 + ΔR2) R 5′-GGCCGCGGTACCTCGAGTTAGTATTCCCTTGAATCCTTCC-3′
SFPQ F 5′-CGGTCGACCATGTCTCGGGATCGGTTC-3′ pCMV-Flag-SFPQ
SFPQ R 5′-CGGGGTACCCTAAAATCGGGGTTTTTT-3′
Primers for qRT-PCR
 NONO F 5′-CTAGCGGAGATTGCCAAAGTG-3′  
 NONO R 5′-GTTCGTTGGACACATACTGAGG-3′  
 ACLY F 5′-ATCGGTTCAAGTATGCTCGGG-3′  
 ACLY R 5′-GACCAAGTTTTCCACGACGTT-3′  
 NEAT1_2 F 5′-CTAGAGGCTCGCATTGTGTG-3′  
 NEAT1_2 R 5′-GCCCACACGAAACCTTACAT-3′  
 GAPDH F 5′-TCAACAGCAACTCCCACTCTTCCA-3′  
 GAPDH R 5′-ACCCTGTTGCTGTAGCCGTATTCA-3′  
The sequences of siRNAs
 si-NONO 5′-CAGGCGAAGUCUUCAUUCA-3′  
 si-ACLY 5′-GAUCAAACGUCGUGGAAAAUU-3′  
 si-NEAT1_2 5′-GGAGGAGUCAGGAGGAAUAUU-3′  
 si-IGF2BP1 5′-CCUGGCUGCUGUAGGUCUU-3′  
 Scrambled siRNA 5′-UUCUCCGAACGUGUCACGUTT-3′  
Primers used for ChIP
 ACLY promoter (0–0.5 k) F 5′-GCTGGGATTACAGGCATGAGCCA-3′  
 ACLY promoter (0–0.5 k) R 5′-GACTACAGGAGCATGCCACC-3′  
 ACLY promoter (0.5–1 k) F 5′-ATAAGATCTAGCCCCAGCTAAGTG-3′  
 ACLY promoter (0.5–1 k) R 5′-CAAGAATCGCTTGAACCCGG-3′  
 ACLY promoter (1–1.5 k) F 5′-GGCCCGAAGTCCACCGTGCCG-3′  
 ACLY promoter (1–1.5 k) R 5′-CGGACCTCACCAAGGCAGGC-3′  
 ACLY promoter (1.5–2 k) F 5′-CGGGTTCGGGCCCCGGCTCGG-3′  
 ACLY promoter (1.5–2 k) R 5′- CGGGGATCTCTGCAATGGA-3′  
Back to article page