Primers for NONO constructs
|
NONO F
|
5′-GCCATGGAGGCCCGAATTCGGATGCAGAGTAATAAAACTTTTAACT-3′
|
pCMV-Myc-NONO
|
NONO R
|
5′-GGCCGCGGTACCTCGAGTTAGTATCGGCGACGTTTGTTTG-3′
|
N terminal deletion of NONO (ΔN) F
|
5′-GCCATGGAGGCCCGAATTCGGCGTCTTTTTGTGGGAAATCT-3′
|
pCMV-Myc-NONO ΔN
|
N terminal deletion of NONO (ΔN) R
|
5′-GGCCGCGGTACCTCGAGTTAGTATCGGCGACGTTTGTTTG-3′
|
C terminal deletion of NONO (ΔC) F
|
5′-GCCATGGAGGCCCGAATTCGGATGCAGAGTAATAAAACTTTTAACT-3′
|
pCMV-Myc-NONO ΔC
|
C terminal deletion of NONO (ΔC) R
|
5′-GGCCGCGGTACCTCGAGTTAGTATTCCCTTGAATCCTTCC-3′
|
DHBS domain of NONO (DHBS) F
|
5′-GCCATGGAGGCCCGAATTCGGCGTCTTTTTGTGGGAAATC-3′
|
pCMV-Myc-NONO DHBS
|
DHBS domain of NONO (DHBS) R
|
5′-GGCCGCGGTACCTCGAGTTAGTATTCCCTTGAATCCTTCC-3′
|
RRM1 deletion of NONO DHBS domain (DHBSΔR1) F
|
5′-GCCATGGAGGCCCGAATTCGGTCCCTTACAGTTCGAAACCT-3′
|
pCMV-Myc-NONO DHBSΔR1
|
RRM1 deletion of NONO DHBS domain (DHBSΔR1) R
|
5′-GGCCGCGGTACCTCGAGTTAGTATTCCCTTGAATCCTTCC-3′
|
Both RRM1 and RRM2 deletion of NONO DHBS domain (DHBSΔR1 + ΔR2) F
|
5′-GCCATGGAGGCCCGAATTCGGTTAGATGATGAAGAGGGAC-3′
|
pCMV-Myc-NONO DHBSΔR1 + ΔR2
|
Both RRM1 and RRM2 deletion of NONO DHBS domain (DHBSΔR1 + ΔR2) R
|
5′-GGCCGCGGTACCTCGAGTTAGTATTCCCTTGAATCCTTCC-3′
|
SFPQ F
|
5′-CGGTCGACCATGTCTCGGGATCGGTTC-3′
|
pCMV-Flag-SFPQ
|
SFPQ R
|
5′-CGGGGTACCCTAAAATCGGGGTTTTTT-3′
|
Primers for qRT-PCR
|
NONO F
|
5′-CTAGCGGAGATTGCCAAAGTG-3′
| |
NONO R
|
5′-GTTCGTTGGACACATACTGAGG-3′
| |
ACLY F
|
5′-ATCGGTTCAAGTATGCTCGGG-3′
| |
ACLY R
|
5′-GACCAAGTTTTCCACGACGTT-3′
| |
NEAT1_2 F
|
5′-CTAGAGGCTCGCATTGTGTG-3′
| |
NEAT1_2 R
|
5′-GCCCACACGAAACCTTACAT-3′
| |
GAPDH F
|
5′-TCAACAGCAACTCCCACTCTTCCA-3′
| |
GAPDH R
|
5′-ACCCTGTTGCTGTAGCCGTATTCA-3′
| |
The sequences of siRNAs
|
si-NONO
|
5′-CAGGCGAAGUCUUCAUUCA-3′
| |
si-ACLY
|
5′-GAUCAAACGUCGUGGAAAAUU-3′
| |
si-NEAT1_2
|
5′-GGAGGAGUCAGGAGGAAUAUU-3′
| |
si-IGF2BP1
|
5′-CCUGGCUGCUGUAGGUCUU-3′
| |
Scrambled siRNA
|
5′-UUCUCCGAACGUGUCACGUTT-3′
| |
Primers used for ChIP
|
ACLY promoter (0–0.5 k) F
|
5′-GCTGGGATTACAGGCATGAGCCA-3′
| |
ACLY promoter (0–0.5 k) R
|
5′-GACTACAGGAGCATGCCACC-3′
| |
ACLY promoter (0.5–1 k) F
|
5′-ATAAGATCTAGCCCCAGCTAAGTG-3′
| |
ACLY promoter (0.5–1 k) R
|
5′-CAAGAATCGCTTGAACCCGG-3′
| |
ACLY promoter (1–1.5 k) F
|
5′-GGCCCGAAGTCCACCGTGCCG-3′
| |
ACLY promoter (1–1.5 k) R
|
5′-CGGACCTCACCAAGGCAGGC-3′
| |
ACLY promoter (1.5–2 k) F
|
5′-CGGGTTCGGGCCCCGGCTCGG-3′
| |
ACLY promoter (1.5–2 k) R
|
5′- CGGGGATCTCTGCAATGGA-3′
| |