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Table 1 Primers used in this study (F, forward; R, reverse)

From: NONO promotes hepatocellular carcinoma progression by enhancing fatty acids biosynthesis through interacting with ACLY mRNA

Name

Sequence

 

Primers for NONO constructs

 NONO F

5′-GCCATGGAGGCCCGAATTCGGATGCAGAGTAATAAAACTTTTAACT-3′

pCMV-Myc-NONO

 NONO R

5′-GGCCGCGGTACCTCGAGTTAGTATCGGCGACGTTTGTTTG-3′

 N terminal deletion of NONO (ΔN) F

5′-GCCATGGAGGCCCGAATTCGGCGTCTTTTTGTGGGAAATCT-3′

pCMV-Myc-NONO ΔN

 N terminal deletion of NONO (ΔN) R

5′-GGCCGCGGTACCTCGAGTTAGTATCGGCGACGTTTGTTTG-3′

 C terminal deletion of NONO (ΔC) F

5′-GCCATGGAGGCCCGAATTCGGATGCAGAGTAATAAAACTTTTAACT-3′

pCMV-Myc-NONO ΔC

 C terminal deletion of NONO (ΔC) R

5′-GGCCGCGGTACCTCGAGTTAGTATTCCCTTGAATCCTTCC-3′

 DHBS domain of NONO (DHBS) F

5′-GCCATGGAGGCCCGAATTCGGCGTCTTTTTGTGGGAAATC-3′

pCMV-Myc-NONO DHBS

 DHBS domain of NONO (DHBS) R

5′-GGCCGCGGTACCTCGAGTTAGTATTCCCTTGAATCCTTCC-3′

 RRM1 deletion of NONO DHBS domain (DHBSΔR1) F

5′-GCCATGGAGGCCCGAATTCGGTCCCTTACAGTTCGAAACCT-3′

pCMV-Myc-NONO DHBSΔR1

 RRM1 deletion of NONO DHBS domain (DHBSΔR1) R

5′-GGCCGCGGTACCTCGAGTTAGTATTCCCTTGAATCCTTCC-3′

 Both RRM1 and RRM2 deletion of NONO DHBS domain (DHBSΔR1 + ΔR2) F

5′-GCCATGGAGGCCCGAATTCGGTTAGATGATGAAGAGGGAC-3′

pCMV-Myc-NONO DHBSΔR1 + ΔR2

 Both RRM1 and RRM2 deletion of NONO DHBS domain (DHBSΔR1 + ΔR2) R

5′-GGCCGCGGTACCTCGAGTTAGTATTCCCTTGAATCCTTCC-3′

SFPQ F

5′-CGGTCGACCATGTCTCGGGATCGGTTC-3′

pCMV-Flag-SFPQ

SFPQ R

5′-CGGGGTACCCTAAAATCGGGGTTTTTT-3′

Primers for qRT-PCR

 NONO F

5′-CTAGCGGAGATTGCCAAAGTG-3′

 

 NONO R

5′-GTTCGTTGGACACATACTGAGG-3′

 

 ACLY F

5′-ATCGGTTCAAGTATGCTCGGG-3′

 

 ACLY R

5′-GACCAAGTTTTCCACGACGTT-3′

 

 NEAT1_2 F

5′-CTAGAGGCTCGCATTGTGTG-3′

 

 NEAT1_2 R

5′-GCCCACACGAAACCTTACAT-3′

 

 GAPDH F

5′-TCAACAGCAACTCCCACTCTTCCA-3′

 

 GAPDH R

5′-ACCCTGTTGCTGTAGCCGTATTCA-3′

 

The sequences of siRNAs

 si-NONO

5′-CAGGCGAAGUCUUCAUUCA-3′

 

 si-ACLY

5′-GAUCAAACGUCGUGGAAAAUU-3′

 

 si-NEAT1_2

5′-GGAGGAGUCAGGAGGAAUAUU-3′

 

 si-IGF2BP1

5′-CCUGGCUGCUGUAGGUCUU-3′

 

 Scrambled siRNA

5′-UUCUCCGAACGUGUCACGUTT-3′

 

Primers used for ChIP

 ACLY promoter (0–0.5 k) F

5′-GCTGGGATTACAGGCATGAGCCA-3′

 

 ACLY promoter (0–0.5 k) R

5′-GACTACAGGAGCATGCCACC-3′

 

 ACLY promoter (0.5–1 k) F

5′-ATAAGATCTAGCCCCAGCTAAGTG-3′

 

 ACLY promoter (0.5–1 k) R

5′-CAAGAATCGCTTGAACCCGG-3′

 

 ACLY promoter (1–1.5 k) F

5′-GGCCCGAAGTCCACCGTGCCG-3′

 

 ACLY promoter (1–1.5 k) R

5′-CGGACCTCACCAAGGCAGGC-3′

 

 ACLY promoter (1.5–2 k) F

5′-CGGGTTCGGGCCCCGGCTCGG-3′

 

 ACLY promoter (1.5–2 k) R

5′- CGGGGATCTCTGCAATGGA-3′