Fig. 2
From: CircRNA_104889 promotes lung adenocarcinoma cell invasion via sponging miR4458
Downregulation of circRNA_104889 in vitro. a Three siRNAs targeting the circRNA_104889 in junction sequences (the red arrows point to the head-to-tail splicing site) were designed. b The expression level of circRNA_104889 in A549 cells after transfection with three siRNAs. c Expression of circRNA_104889 after siRNA2 transfection for 24, 48, and 72 h in A549 cells. d The mRNA levels of the parental gene PAPPA after knockdown of circRNA_104889 with siRNA2. Data are presented as mean ± SD from 3 independent experiments in triplicates, n = 3. Significant effect of the treatments, *p < 0.05, **p < 0.01, ***p < 0.001