Skip to main content
Fig. 3 | Cancer Cell International

Fig. 3

From: Identification of circular RNA hsa_circ_0044556 and its effect on the progression of colorectal cancer

Fig. 3

Has_circ_0044556 is a circRNA. a Verification that has_circ_0044556 is a circRNA, using divergent and convergent primers. Top, schematic illustration of has_circ_0044556 locus with specific primers. Bottom, RT-PCR products with divergent primers showing circularization of has_circ_0044556 gDNA, genomic DNA. Middle, Sanger sequencing result of has_circ_0044556. b RT-PCR products with Rnase R treatment showing circularization of has_circ_0044556. c The expression levels of has_circ_0044556 and GAPDH in the presence or absence of Rnase R treatment, respectively. d schematic illustration showed three targeted siRNAs. SiRNA targets the back-splice junction of has_circ_0044556. e, f qRT-PCR for has_circ_0044556 in CRC cells treated with three siRNAs. g The expression levels of COL1A1 mRNA treated with siRNA-3

Back to article page