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Fig. 2 | Cancer Cell International

Fig. 2

From: MicroRNA-585 inhibits human glioma cell proliferation by directly targeting MDM2

Fig. 2

miR-585 inhibits proliferation of human glioma cells in vitro. ac U251 and A172 cells were stably infected with lentivirus expressing empty vector (LV-vec) or miR-585 (LV-miR-585). a miR-585 expression was determined by qRT-PCR analysis. U6 snRNA was used as an internal control. Data are mean ± SD (n = 3). Two-tailed unpaired Student’s t-test. **P < 0.01. b The cell number of U251 and A172 cells cultured for consecutive 3 and 6 days was counted by CCK-8 assay. Data are mean ± SD (n = 5). One-way ANOVA followed by post hoc Bonferroni test. **P < 0.01 (vs. LV-vec). c The proliferating U251 and A172 cells were stained with EdU, and the percentage of EdU positive cells was analyzed by FACS assay. Data are mean ± SD (n = 5). Two-tailed unpaired Student’s t-test. **P < 0.01. df U251 and A172 cells were stably infected with shRNA-vec or shRNA-miR-585. The expression of miR-585 (d), cell number of U251 cells as assayed by CCK-8 (e) and EdU incorporation (f) were analyzed as same as in (ac)

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