Fig. 3

miR-585 inhibits growth of human glioma cells in vivo. a–d U251 cells stably infected with LV-vec or LV-miR-585 were intracranially inoculated in athymic nude (nu/nu) mice (n = 7 mice per group). a Representative T₂-weighted imaging (T₂WI) of mice with xenografted glioma after 2 weeks of inoculation was captured using 3.0 T MRI. b The signal intensity of T₂WI as shown in (a) in each group was analyzed. Data are mean ± SD (n = 7). Two-tailed unpaired Student’s t-test. **P < 0.01. c miR-585 expression in isolated tumors was determined by qRT-PCR analysis. U6 snRNA was used as an internal control. Data are mean ± SD (n = 3). Two-tailed unpaired Student’s t-test. **P < 0.01. d Ki-67 expression in U251 tumor sections was analyzed by immunohistochemical (IHC) staining. The representative images (left) and percentage of Ki-67 positive cells (right) are shown. Twelve random fields from 3 sections of each group were quantified. Data are mean ± SD (n = 3). Two-tailed unpaired Student’s t-test. **P < 0.01