Fig. 5

MDM2 restoration rescues inhibitory effect of miR-585 on human glioma cell proliferation. a–c U251 cells stably infected with LV-vec or LV-miR-585 were transfected with pcDNA3.1 vector or pcDNA3.1 MDM2. a The protein expression of MDM2 was determined by Western blot analysis. β-Actin was used as a loading control. The representative images from 3 independent experiments are shown. b The cell number of U251 cells cultured for consecutive 3 and 6 days was counted by CCK-8 assay. Data are mean ± SD (n = 5). One-way ANOVA followed by post hoc Bonferroni test. **P < 0.01; *P < 0.05. c The proliferating U251 cells were stained with EdU. The percentage of EdU positive cells was analyzed by FACS assay. Data are mean ± SD (n = 5). Two-tailed unpaired Student’s t-test. **P < 0.01; *P < 0.05. d–f A172 cells stably infected with LV-vec or LV-miR-585 were transfected with pcDNA3.1 vector or pcDNA3.1 MDM2. The protein expression of MDM2 (d), cell number of A172 cells as assayed by CCK-8 (e) and EdU incorporation (f) were analyzed as in (a–c)