Fig. 12

miR-221-3p inhibits KIT expression in PC cells. a Direct binding sequences of miR-221-3p and KIT mRNA. b–e Luciferase activities of 3’UTR KIT-luc constructs in PC cells after transfection with mimic, inhibitor or control. The results were assessed with t tests (unpaired). f, g KIT expression after transfection with mimic, inhibitor or control determined by RT-qPCR. h–k KIT expression after transfection with mimic, inhibitor or control determined by western blotting